Physical Properties of Proteins—Student Laboratory Kit

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Structure determines function—this is one of the most universal themes in biochemistry. What factors influence the physical properties of proteins and determine their structure and function? In this hands-on student laboratory kit, students examine the effects of acids and bases, inorganic salts, organic solvents and temperature on the physical properties of proteins. The dramatic results are visually impressive and allow students to see for themselves how sensitive proteins are to physiological conditions and to the addition of external agents. Students learn how these factors affect the interactions among amino acid side chains that are responsible for the stable three-dimensional structures of proteins—and thus change the actual shapes of proteins. Students observe that some changes are easily reversed and thus relatively harmless while other changes are essentially irreversible and lead to complete loss of protein function. The definition and applications of protein denaturation are also explored. Includes reproducible student handouts, detailed background information, Teacher Notes with sample data and answers to all questions and all necessary chemicals and consumable supplies.

Complete for 30 students working in pairs.